Isolation and Identification of Listeria Species

Isolation and Identification of Listeria Species Alesha Coleman-McCallum Title: Isolation and Identification of Listeria species from chicken sample using Palcam broth (pre-enrichment), UVM 11 broth (selective enrichment), Palcam and Oxford agars (selective plating) also confirmation using biochemical tests. Objectives: To isolate Listeria species from chicken sample To observe the reaction of listeria on selective medium To confirm the Listeria species using biochemical tests Introduction Listeria is a genus of aerobic parasitic, gram positive rod-shaped bacterium (Define, n.d). This genus has more than 10 species with the commonly encountered being: Listeria monocytogenes, Listeria innocua, Listeria ivanovii, Listeria welshimeri, Listeria seeligeri, Listeria grayi, Listeria murrayi. Members of this genus are extensively spread in the environment and maybe found in soil, plants, gastrointestinal tract of animal and humans. Listeria monocytogenes species is of great concern because it is pathogenic to humans and causes Listeriosis. Listeriosis is a foodborne illness (Hardy Diagnostics, 1996). Listeria monocytogenes is different from most bacteria since it can grow in the cold, salt, acid and air-tight conditions. The increased demand of ready to eat foods especially in first world countries has the potential of listeriosis more eminent. Pregnant women, older adults, young children and immunocompromised persons are more susceptible to Listeriosis infections. Therefore cooking, pasteurization/applying heating steps to food, avoiding raw meat and milk/ moist or processed food and washing hands regularly may reduce the risk of infections. (FDA, 2004). According to Food Quality Safety USA [4] a dry and clean work environment is crucial in avoiding listeria outbreaks in the food industry. Methods for Identification Methods used in BI208 lab for identification of Listeria were Palcam broth (pre-enrichment) then UVM 11 broth for selective enrichment step, Palcam (selective agent) and Oxford agars (selective and differential agent). For confirmation of species: organism was plated on a blood agar to check for hemolysis. Biochemical test included SIM tube for motility, mannitol, D Xylose and L Rhamnose reactions obtained. Other methods that could be used to identify listeria species are: Polymerase Chain Reaction (PCR), Rapid Identification Kits and Serological tests (MFHPB-07, 2012). Results Table showing results obtained from Listeria media reaction Media Observations Reaction Palcam Agar Shiny, smooth, circular, convex colonies. No fermentation No Hydrolsis Oxford Agar Black colour around colonies Esculin Reduction Blood Cream, hilly,glistening colonies No Beta-hemolysis Mannitol Transparent colonies Negative D Xylose Transparent colonies Negative L Rhamnose Light yellow fermentation around colonies Positive SIM Tube Umbrella shaped growth Positive Gram Stain gram positive rods Assuming Listeria monocytogenes control was used: Media Expected Results Palcam Broth Cloudy UVM 11 Cloudy Palcam Agar No fermentation Positive Hydrolysis Oxford Agar Esculin Reduction Blood Beta-hemolysis Mannitol Negative D Xylose Negative L Rhamnose Positive SIM Tube Positive Discussion Listeria innocua was isolated from the chicken sample. Listeria innocua is not usually implicated in food born-illnesses however an isolated death in an elderly patient was reported (Perrin, Bemer and Delamare, 2003). Portions of chicken sample were first placed in pre-enrichment broth in order for stressed cells to become viable and all other bacterial cells to multiply.   Enrichment stage is to partially suppress unwanted organisms and allow listeria to thrive. Oxford agar (OXA) is both selective and differential. The selective properties of OXA agar will not allow gram negative organisms to grow while suppressing most gram positive organisms (Oxoid, n.d). The different property of OXA agar will allow some species of Listeria to be totally inhibited, growth with or without blackened colonies (MFHPB-07, 2012). Palcam agar   utilizes two indicator systems: esculin and mannitol.   Listeria monocytogenes changes esculin to a black complex around colonies but does not ferment mannitol. Therefore some gram positive organisms such as enterococci and staphylococci will ferment mannitol hence can be ruled out as contaminants (Oxoid, n.d). Blood agar was used to determine if the organ ism is beta-hemolytic. Other Biochemical tests were mannitol, D Xylose, L Rhamnose and SIM Tube to differentiate and confirm the species. Since Listeria monocytogenes is pathogenic to humans a 2 Class Sampling Plan would be used to asses if the chicken is fit for consumption. Different species of an organism may exhibit variable characteristics and maybe mistaken. Listeria species were found and should be deemed unacceptable for consumption. Further testing such as PCR which is very specific could be used to conclusively identify the species present. Conclusions Based on the objectives previously outlined, isolation and Identification of Listeria species from chicken sample using Palcam broth method was very effective. References Define,n.d. Listeria Genus. Retrieved February 22, 2017 from http://www.dictionary.com/browse/listeria Hardy Diagnostics, 1996. Food Borne-illneses Retrieved February 22, 2017 from https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/Listeria.htm FDA, 2004. Food-Borne Pathogenic Microorganisms and Natural Toxins Handbook: The Bad Bug Book U.S. FDA/CFSAN. Center for Food Safety and Applied Nutrition, Food and Drug Administration, College park, MD. Food Quality Safety (formerly Food Quality),2007.   Retrieved February 20, 2017 from http://www.foodqualityandsafety.com/article/prevent-and-control-listeria Jay, J.M.,Loessner, M.J. Golden, D. A. (2005). Modern Food Microbiology: Indicator of Food Microbial Quality and Safety, 476-477. United States of America: Springer Science+Business Media LLC . MFHPB-07 Method, November, 2012. The isolation of Listeria monocytogenes and other listeria species from food and environment samples using Palcam broth. Retrieved February 20, 2017 from  http://www.hc-sc.gc.ca/fn-an/res-rech/analy-meth/microbio/volume2-eng.php Monique Perrin, Michel Bemer and Catherine Delamare, 2003. Fatal Case of Listeria innocua Bacteremia: Journal of Clinical Microbiology Oxoid, n.d. Enrichment Media for Listeria. Retrieved February 20, 2017 from  http://www.oxoid.com/uk/blue/prod_detail/prod_detail.asp?pr=CM0856org=91c=uklang=EN US Government, n.d. Retrieved February 20, 2017 from https://www.foodsafety.gov/poisoning/causes/bacteriaviruses/listeria/ Zimbro, M.J., Power D.A., Miller,M.M., Wilson, G.E., Johnson, J.A., (Revised 2009). Difco BBL Manual: Palcam and Oxford Agar, Culture Media for Specific Groups of Microorganisms. United States of America: Becton, Dickinson and Company.

Hate Crimes and The Mitchell v. Wisconsin Decision Essay -- Hate Crime

Hate Crimes and The Mitchell v. Wisconsin Decision The American Heritage Dictionary defines hate as intense dislike or animosity. However, defining hate as the basis for a crime is not as easy without possibly jeopardizing constitutional rights in the process. Hate crime laws generally add enhanced punishments to existing statues. A hate crime law seeks to treat a crime, if it can be demonstrated that the offense was a hate crime differently from the way it would be treated under ordinary criminal law. Since the 1980s, the problem of hate crimes has attracted increasing research attention, especially from criminologists and law enforcement personnel who have focused primarily on documenting the prevalence of the problem and formulation criminal justice responses to it. Lawmakers have passed legislation to encourage data collection and attach enhanced penalties to hate crimes at both state and federal levels.   Ã‚  Ã‚  Ã‚  Ã‚  When Americans are assaulted merely because of their real or perceived sexual orientation, gender, or disability, the law should be as tough on their assailants as it currently is tough on criminals who attack based on racial, religious, or ethnic bias. Yet only in rare circumstances can the federal government investigate and prosecute hate violence against gays, lesbians, or bisexuals. Attempts have been made to reach a definition of hate crime, including that it is a crime, most commonly violence, motivated by prejudice, bias or hatred towards a particular group of which the victim is rarely significant to the offender and is most commonly a stranger to him or her. The current law (18 U.S.C. 245) permits federal prosecution of a hate crime only if the crime was motivated by bias based on race, religion, national origin, or color, and the assailant intended to prevent the victim from exercising a "federally protected right" (e.g. voting, attending school, etc.) T his dual requirement substantially limits the potential for federal assistance in investigating or prosecuting hate crimes, even when the crime is particularly heinous.   Ã‚  Ã‚  Ã‚  Ã‚  Hate crimes demand a priority response because of their special emotional and psychological impact on the victim and the victims’ community. The damage done by hate crimes cannot be measured solely in terms of physical injury or dollars and cents. Hate crimes may effectively intimidate other members of the vi... ...  Ã‚  Ã‚  Ã‚  Law." Human Rights 22 (1995): 32-33 Dennis, Valerie. MTV remembers Matthew Shepard with 17-hour program on hate   Ã‚  Ã‚  Ã‚  Ã‚  crimes, University Wire, 01-10-2001 Feingold, Stanley. "Hate Crime Legislation Muzzles Free Speech." The National Law  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Journal 15 (July 1, 1993): 6, 16 Franke-Folstad, Kim. Denver Rocky Mountain News Staff Writer, HATE-CRIME   Ã‚  Ã‚  Ã‚  Ã‚  LAWS NOT A BLACK-WHITE ISSUE. Denver Rocky Mountain News, 01-18-  Ã‚  Ã‚  Ã‚  Ã‚  1999, pp 6A Gellman, Susan. "Sticks And Stones." UCLA Law Review 39 (December, 1991):  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  333-396 Patrick, Robert F. Cops find hate often has broad definition, The Washington Times, 04-  Ã‚  Ã‚  Ã‚  Ã‚  02-01, pp C1 R.A.V. v. St. Paul (505 U.S. 377) Texas v. Johnson (491 U.S. 397) The Associated Press, Reno Fights Hate Crimes, Newsday, 01-09-1998, pp A21 The Christian Science Publishing Society 30 Brad Knickerbockers, Staff writing of The   Ã‚  Ã‚  Ã‚  Ã‚  Christian Science Monitor, Hate Crimes: Should they receive special attention?   Ã‚  Ã‚  Ã‚  Ã‚  The Christian Science Monitor, 06-23-200, pp 22 U.S. v. O'Brien (391 U.S. 367) Wisconsin v. Mitchell (508 U.S. 476) Wooley v. Maynard (430 U.S. 705) W.V. State Board of Education v. Barnette (319 U.S. 624)

The Monster Inside Me

I had everything. Loving family, supportive friends, good grades, degree in ballet†¦ etc. Not much, but there’s no shortage for anything. But life isn’t always perfect, especially when the monster inside you starts to show. I was at the dance studio half an hour earlier before the rehearsal starts. Determined to be the best as I got the first role for the upcoming ballet production â€Å"Swan Lake†. I got all the applause and compliments from all the musical directors and choreographers. Everything was meant to be, I was the brightest star. But life isn’t always fair.The door cracked open as I was doing my last stretching before heading to the stage. The director walked in, along with another girl from the ensemble group. â€Å"Charlotte,† She said. â€Å"She will take the lead role from now on, you’ll be her backup,† the director continued. â€Å"Five minutes on stage. † They went out the door. I was stunned. Everything ha ppened in just a blink of an eye. I worked so hard for this role and everything was meant to be. I couldn’t accept the fact that someone is better than me. Someone that can make the choreographers and directors think for a second thought.I wasn’t satisfied. I went up the stage and argued with the director. Thinking of something that could make him change his decision. â€Å"Your figure is not what we want for the role. † I was beaten by that. Never in my life did someone actually point out my imperfection before. I thought I was perfect. I rushed to the toilet to hide my tears. I am strong, or in the other word, I was afraid to lose. High self-esteem is what I got since the day I was born. I highly believe that I was worth everything because I spent much of my time striving to achieve perfection in every aspect of my life.What I did not realize was that in my desperate need to be perfect, I sacrificed the very body and mind that allowed me to live. I looked at m yself in the mirror. The reflection of mine started to twist. I saw an ugly, worthless and hopeless girl. Her empty eyes weren’t showing anything but angers. Her body began to grow bigger and bigger. She seemed to be the brightest among all, but when the darkness comes, she’s nothing but an empty shell. From that day onwards, my life changed. I was never happy before. Jealousy was what controlling me. I felt very inadequate and unsuccessful.I started to believe that my life was a failure. And what had caused it like this is no doubt but my weight. I had always been an average size. But I was convinced by my inner voice that I was overweight. I found myself involved in a competition again. But this time, I was competing against myself. I started of with a normal diet. Thought I’ll just lose a few pounds and everything will be okay. I was wrong. When the first pound was lost, I was addicted to the numbers on the scales. My mind became obsessed with beating my body at this game. Though I know I’m slowly killing myself, but jealousy was my biggest motivation.I was dying to win my pride back and prove them wrong. I slowly cut back on what I eat each day. With every portion I didn’t finish or meal I skipped, I told myself that I was succeeding, and in turn, I felt good about myself. That was my darkest secret. I still join my family dinner. Just to avoid my family to notice my odd behaviour. But I felt disgusted and useless after every meal. I was then introduced to purging. Purging had sort of changed my life because I could eat what I want and what I had to do is just purge them out afterwards.My parents had no idea that their loving and caring daughter is a total different soul now. Every time I finished meal I will make a beautiful excuse to my room. They had no idea what is happening behind that door. Toilet bowl and icy tiles was what I face every night. My reflection from the mirror is nothing but an empty soul. My eyes were like two empty holes burning in flames. I just want my role back. My inner voice told me that it will be worth it every time. There, I slide two fingers down my throat and began to purge out everything.Not just the food I ate, but every bit of my soul. The monster inside me had taken over everything I had, purging out my original self. My mind was possessed by jealousy, anger and desperation, and I couldn’t help it. I was lost and desperately searching for the light at the end of the tunnel. Even so, I had never gave up my hope on ballet. I lost a lot of weight, losing more that I could have imagined. I just couldn’t control myself, my soul was lost, life was empty and I couldn’t figure out why. But my spirits were high and I was still so motivated and determined to get my role back.I knew I still had to convince the directors that I was the main star. I saw my reflection on the mirror, I saw the beautiful me. One day before the show, I was on the backstage. An xiously waiting for the right time like lion waiting for its prey. There she came, going down the stairs. My eyes were flaming. Jealousy had driven me to my limits, the limits where I could do everything just to get my role back. I have to vanish her. I was possessed by the monster inside me, I ran towards her in light speed and pushed her off the stairs with the last energy I reserved.My heart was racing. She was admitted to the hospital. I have no regrets on what I’ve done. Never in my life that I was so happy by the feeling of winning. But I wasn’t me anymore, the bright and loving girl had completely turned into a devil. The feeling of snatching back of what belongs to me was what pleased me. I was insane. Standing on the stage, the lights were on me. I gave a revengeful smile to the directors and as soon as I finished my last step, I collapsed. Heart attack hits me. The sounds of sirens were apparent and soon after, I noticed a medic trying to resuscitate me.I kne w my time was done, my heart was failing. It was beating weaker. â€Å"Perfection, I finally found it. It was perfect. † I saw my body, it was lying down on the ground like a doll. There were people mourning and weeping. At first, I couldn’t understand why. I saw more and more people, the people whom I recognized came over, filled with unbearable thoughts of sadness and grief. I knew then I was gone, I was no longer a part of this world. I thought I did what I had to do. In the end, I defeated the purpose and paid it with my life. The Monster Inside Me I had everything. Loving family, supportive friends, good grades, degree in ballet†¦ etc. Not much, but there’s no shortage for anything. But life isn’t always perfect, especially when the monster inside you starts to show. I was at the dance studio half an hour earlier before the rehearsal starts. Determined to be the best as I got the first role for the upcoming ballet production â€Å"Swan Lake†. I got all the applause and compliments from all the musical directors and choreographers. Everything was meant to be, I was the brightest star. But life isn’t always fair.The door cracked open as I was doing my last stretching before heading to the stage. The director walked in, along with another girl from the ensemble group. â€Å"Charlotte,† She said. â€Å"She will take the lead role from now on, you’ll be her backup,† the director continued. â€Å"Five minutes on stage. † They went out the door. I was stunned. Everything ha ppened in just a blink of an eye. I worked so hard for this role and everything was meant to be. I couldn’t accept the fact that someone is better than me. Someone that can make the choreographers and directors think for a second thought.I wasn’t satisfied. I went up the stage and argued with the director. Thinking of something that could make him change his decision. â€Å"Your figure is not what we want for the role. † I was beaten by that. Never in my life did someone actually point out my imperfection before. I thought I was perfect. I rushed to the toilet to hide my tears. I am strong, or in the other word, I was afraid to lose. High self-esteem is what I got since the day I was born. I highly believe that I was worth everything because I spent much of my time striving to achieve perfection in every aspect of my life.What I did not realize was that in my desperate need to be perfect, I sacrificed the very body and mind that allowed me to live. I looked at m yself in the mirror. The reflection of mine started to twist. I saw an ugly, worthless and hopeless girl. Her empty eyes weren’t showing anything but angers. Her body began to grow bigger and bigger. She seemed to be the brightest among all, but when the darkness comes, she’s nothing but an empty shell. From that day onwards, my life changed. I was never happy before. Jealousy was what controlling me. I felt very inadequate and unsuccessful.I started to believe that my life was a failure. And what had caused it like this is no doubt but my weight. I had always been an average size. But I was convinced by my inner voice that I was overweight. I found myself involved in a competition again. But this time, I was competing against myself. I started of with a normal diet. Thought I’ll just lose a few pounds and everything will be okay. I was wrong. When the first pound was lost, I was addicted to the numbers on the scales. My mind became obsessed with beating my body at this game. Though I know I’m slowly killing myself, but jealousy was my biggest motivation.I was dying to win my pride back and prove them wrong. I slowly cut back on what I eat each day. With every portion I didn’t finish or meal I skipped, I told myself that I was succeeding, and in turn, I felt good about myself. That was my darkest secret. I still join my family dinner. Just to avoid my family to notice my odd behaviour. But I felt disgusted and useless after every meal. I was then introduced to purging. Purging had sort of changed my life because I could eat what I want and what I had to do is just purge them out afterwards.My parents had no idea that their loving and caring daughter is a total different soul now. Every time I finished meal I will make a beautiful excuse to my room. They had no idea what is happening behind that door. Toilet bowl and icy tiles was what I face every night. My reflection from the mirror is nothing but an empty soul. My eyes were like two empty holes burning in flames. I just want my role back. My inner voice told me that it will be worth it every time. There, I slide two fingers down my throat and began to purge out everything.Not just the food I ate, but every bit of my soul. The monster inside me had taken over everything I had, purging out my original self. My mind was possessed by jealousy, anger and desperation, and I couldn’t help it. I was lost and desperately searching for the light at the end of the tunnel. Even so, I had never gave up my hope on ballet. I lost a lot of weight, losing more that I could have imagined. I just couldn’t control myself, my soul was lost, life was empty and I couldn’t figure out why. But my spirits were high and I was still so motivated and determined to get my role back.I knew I still had to convince the directors that I was the main star. I saw my reflection on the mirror, I saw the beautiful me. One day before the show, I was on the backstage. An xiously waiting for the right time like lion waiting for its prey. There she came, going down the stairs. My eyes were flaming. Jealousy had driven me to my limits, the limits where I could do everything just to get my role back. I have to vanish her. I was possessed by the monster inside me, I ran towards her in light speed and pushed her off the stairs with the last energy I reserved.My heart was racing. She was admitted to the hospital. I have no regrets on what I’ve done. Never in my life that I was so happy by the feeling of winning. But I wasn’t me anymore, the bright and loving girl had completely turned into a devil. The feeling of snatching back of what belongs to me was what pleased me. I was insane. Standing on the stage, the lights were on me. I gave a revengeful smile to the directors and as soon as I finished my last step, I collapsed. Heart attack hits me. The sounds of sirens were apparent and soon after, I noticed a medic trying to resuscitate me.I kne w my time was done, my heart was failing. It was beating weaker. â€Å"Perfection, I finally found it. It was perfect. † I saw my body, it was lying down on the ground like a doll. There were people mourning and weeping. At first, I couldn’t understand why. I saw more and more people, the people whom I recognized came over, filled with unbearable thoughts of sadness and grief. I knew then I was gone, I was no longer a part of this world. I thought I did what I had to do. In the end, I defeated the purpose and paid it with my life.

John Gotti essays

John Gotti essays John Gotti lived in one of those crowded dirty apartment. A young John Gotti seeked an impoverished existence with his parents and eleven sisters and brothers. His father brarely ever worked and when he did only at menial jobs. Also had a problem risking the money that the family did have on gambling. Eventually Gottis dad rasied enough money so the family could move to central Brooklyn, which was known as East New York at the time. Gotti went through alot to get to the top but he made it. Growing up in central brooklyn with nothing to look foward to Gotti seeked out the Cosa Nostra. Gotti was power hungry and needed respect he felt that he could gain them both by being a part of the Cosa Nostra. He started as many young boys did, running errands for the gangsters, molding himself into a young bully with a future. His first major problems with the police occurred when he tried to steal a cement mixer and it fell on his feet this injury affected his future for the rest of his life. He quit school at sixteen and rose to leadership in a local street gang of thieves called the Fulton-Rockaway Boys, named after two streets in their neighborhood. At an early age he exerted his bad temper, dominance and readiness to engage in fistfights. This was just what he needed to develop his potential as Boss of the Moffia. In the mid 1960's, Gotti's boss Carmine Fatico moved his headquarters out to Ozone Park near JFK Airport. Gotti and his brothers, Angelo and Willie became relatively successful hijackers. They were hjacking for about 8 years untill they got caught in 1968 and were in jail untill 1972. When Gotti got out of prison He idmediatly went back to Ozone Park. The headquarters had been renamed on the streets as The Bergin Hunt and Fish Club. Two important things happened in his life to significantly lift his status in the Cosa Nostra. The first was that his boss Carmine Fatico faced a loansharking indictment, so Gotti became...

10 Fascinating Facts About Spiders

10 Fascinating Facts About Spiders Spiders: some people love them, some people hate them. Regardless of if youre an arachnophile or an arachnophobe, youll find these 10 facts about spiders fascinating. 1. Spider Bodies Have Two Parts: A Cephalothorax and an Abdomen All spiders, from tarantulas to jumping spiders, share this common trait. The simple eyes, fangs, palps, and legs are all found on the anterior body region, called the cephalothorax. The spinnerets reside on the posterior region, called the abdomen. The unsegmented abdomen attaches to the cephalothorax by means of a narrow pedicel, giving the spider the appearance of having a waist. 2. With the Exception of One Family, All Spiders Are Venomous Spiders use venom to subdue their prey. The venom glands reside near the chelicerae, or fangs, and are connected to the fangs by ducts. When a spider bites its prey, muscles around the venom glands contract, pushing venom through the fangs and into the animal. Most spider venom paralyzes the prey. The spider family Uloboridae is the only known  exception to this rule. Its members do not possess venom glands. 3. All Spiders Are Predators Spiders hunt and capture prey. The majority feed on other insects and other invertebrates, but some of the largest spiders may prey on vertebrates such as birds. The true spiders of the order Araneae comprise the largest group of carnivorous animals on Earth. 4. Spiders Can't Digest Solid Foods Before a spider can eat its prey, it must turn the meal into a liquid form. The spider exudes digestive enzymes from its sucking stomach onto the victims body. Once the enzymes break down the tissues of the prey, it sucks up the liquefied remains, along with the digestive enzymes. The meal then passes to the spiders midgut, where nutrient absorption occurs. 5. All Spiders Produce Silk Not only can all spiders make silk, but they can do so throughout their life cycles. Spiders use silk for many purposes: to capture prey, to protect their offspring, to assist them as they move, for shelter, and to reproduce (more on that in a moment). Not all spiders use silk the same way. 6. Not All Spiders Spin Webs Most people associate spiders with webs, but some spiders dont construct webs at all. Wolf spiders, for example, stalk and overtake their prey, without the aid of a web. Jumping spiders, which have remarkably good eyesight and move quickly, have no need for webs, either. They simply pounce on their prey! 7. Male Spiders Use Modified Appendages Called Pedipalps to Mate Spiders reproduce sexually, but males use an unusual method to transfer their sperm to a mate. The male first prepares a silk bed or web, onto which he deposits sperm. He then draws the sperm into his pedipalps, a pair of appendages near his mouth, and stores the semen in a sperm duct. Once he finds a mate, he inserts his pedipalp into her genital opening and releases his sperm. 8. Males Risk Being Eaten by Their Female Mates Females are typically larger than their male counterparts. A hungry female may consume any invertebrate that comes along, including her suitors. Male spiders sometimes  use courtship rituals to identify themselves as mates and not meals. Jumping spiders, for example, perform elaborate dances from a safe distance and wait for the females approval before approaching. Male orb weavers (and other web-building species) position themselves on the outer edge of the females web, and gently pluck a thread to transmit a vibration. They wait for a sign that the female is receptive before venturing closer. 9. Spiders Use Silk to Protect Their Eggs Female spiders deposit their eggs on a bed of silk, which they prepare just after mating. Once a female produces the eggs, she covers them with more silk. Egg sacs vary greatly, depending on the type of spider. Cobweb spiders make thick, watertight egg sacs, while cellar spiders use a minimum of silk to encase their eggs. Some spiders produce silk that mimics the texture and color of the substrate on which the eggs are laid, effectively camouflaging the offspring. 10. Spiders Don't Move by Muscle Alone Spiders rely on a combination of muscle and hemolymph (blood) pressure to move their legs. Some joints in spider legs lack extensor muscles entirely. By contracting muscles in the cephalothorax, a spider can increase the hemolymph pressure in the legs, and effectively extend their legs at these joints. Jumping spiders jump using a sudden increase in hemolymph pressure that snaps the legs out and launches them into the air.

History of the United States Essay Example | Topics and Well Written Essays - 1500 words

History of the United States - Essay Example His leadership during the Great Depression besides having utter joblessness natives won the confidence of his subjects. This is because he instituted the policy of banks’ closure that persisted for 100 days to shun the incidences of people losing their homes and enterprises. Studies especially those of Zinn contend that the uprising of democratization the fuelling emanated from the diversity in societal strata (Zinn 10-22). This entailed unequal treatment of the blacks and other races that formed the oppressed society by the whites who were the elites. Primarily, this was after the arrival of the Columbus in the N. America prior the importation of servitude meant to work in the plantations and factories. Hence, resulting to human and dignity degradation that prompted the oppressed to seek their emancipation via unions instituted then to relief the people. Oppression entailed low wages and even enslavement especially by the Southerners where even after the declaration regarding servitude abolition some slaveholders were too reluctant to consent. Additionally, the severity of factory oppression, plantations and mines continued to the 20Th C, which was the great awakening of the masses (Zinn 123). They desired equal treatment like the white; claiming the relation amid them was more of deprivation than that of safeguarding human dignity. This was mostly in the last quarter of the 20Th era where its actions almost characterize the Zinn’s argument even to the current. Upon becoming the president, FDR ordered heavily taxing on the wealthy so that the state would relief the underprivileged by gaining their economic stability. This marked the true source of democracy besides other events triggered later by civil rights’ agitators, for illustration, Malcolm X. Steps to full democratization emerged with the Americans and other natives starting to believe in themselves where they sought all possible ways of their emancipation. This entailed mind emanci pation prior resulting to the physical one, especially abolishing segregation that was evident amid the states. America prior the democratization era, it endured severe segregation where the Southerners compared to other citizens could not enjoy similar rights in public like their white counterparts. This yielded to severe and active retaliations by the segregated group, which entailed the use of diverse ways by the civil rights’ leaders. Southerners and numerous people who encountered segregation devised diverse rights’ movements meant to agitate for their privileges. According to Zinn were mainly farm workers who besides facing discrimination their wages were low, until civil activism came to their aid. This entailed even religious-based associations spearheaded by icons like Martin Luther King. His initial debut was during Montgomery Bus Boycott (1955) where the incidence lasted for 382 days. The incidence emanated from Rose Parker declining to offer a seat to a whi te man, which brewed uproar that culminated to her arrest. Learning of this, Martin organized nonviolent demonstration where the blacks declined utilizing the public transport, which yielded to immense funds loss especially to the buses’ owners. Since then, Martin continued to advocate for peaceful disobedience to the harsh laws that segregated Negros and offered the

Who Is Pepsis Biggest Competitor Term Paper Example | Topics and Well Written Essays - 750 words

Who Is Pepsis Biggest Competitor - Term Paper Example Their products, which include foods, beverages, and snacks, compete with other products from other manufacturers on the grounds of quality, price and their variety of products and their distribution efficiencies. Their success in marketing their products has largely arisen from their success in promoting their existing products, the effectiveness of their advertisement campaigns, their marketing programs, packaging of their products and their pricing strategies (PepsiCo, p.28). Their success has also been facilitated by their increased efficiencies in their production techniques, their trademark and brand development and their protection within the neighborhood, regional and worldwide markets. The company believes that in order for them to be able to compete effectively, their brands and marketing strategies should be strong. They also believe that their innovations and the quality of their products and their flexible distribution channels will allow their company to remain competiti ve in all the markets they deal with (PepsiCo, p.29). The Company uses the straight-line methodology of calculating their depreciation and amortization. According to the company’s 2008 financial reports, the depreciation and amortization of the tangible assets that they own is carried out on a five-year basis. In addition, the company’s 2011 annual financial reports indicate that their country’s tax laws require them to include their depreciation expenses at periodic intervals of five years (PepsiCo, p.36). The Pepsi Company had authorized 3,600 shares of common stock in the year 2011 according to their annual reports.